The Primary Objectives of This Laboratory is to Develop Novel Biocatalysts for White Biotechnology.
教授：原山重明
学位
1976年 理学博士 東京大学
職歴
東京大学理学部助手
スウェーデン ウプサラ大学客員研究員（Research Fellow)
米国 イリノイ大学客員教授（Invited Professor）
スイス ジュネーブ大学医学部助教・講師・准教授・特任教授
(Maitre Assistant/Lecteur/Maitre
d'Enseignement et de Recherche/Chef de
Laboratoire de Biotechnologie)
海洋バイオテクノロジー研究所 室長・副所長・所長・シニアフェロー
独立行政法人 製品評価技術基盤機構 生物遺伝資源開発部門 部門長
中央大学理工学部 生命科学科 教授（現在）
専門
微生物学
研究テーマ
微細藻類を用いた有用物質生産に関する研究
軽油生産能を有する単細胞緑藻の転写因子大量発現による生産性向上の研究開発
担当科目
基礎分子生物学
生命科学英語１
生命科学英語２
遺伝情報学実験
生命科学英語上級
応用生物学
生体物質機能学
バイオテクノロジー概論
研究業績（過去10年）
原著論文Wakai S, Harayama S. Isolation of bacteria rapidly adhering to metal iron surface. Materials Technology: Advanced Biomaterials, 30(B1), B38-B43 (2015).
Matsuwaki I, Harayama S, Kato M. Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea. Algal Res 9:1-7(2015).
Iino T, Ito K, Wakai S, Tsurumaru H, Ohkuma M, Harayama S. Iron Corrosion Induced by Nonhydrogenotrophic Nitrate-Reducing Prolixibacter sp. Strain MIC1-1. Appl Environ Microbiol. 81:1839-1846 (2014).
Wakai S, Ito K, Iino T, Tomoe Y, Mori K, Harayama S. Corrosion of iron by iodide-oxidizing bacteria isolated from brine in an iodine production facility. Microb Ecol. 68:519-527 (2014).
Matsumoto T, Yamamura H, Hayakawa J, Watanabe Y, Harayama S. Influence of extracellular polysaccharides (EPS) produced by two different green unicellular algae on membrane filtration in an algae-based biofuel production process. Water Sci Technol. 69:1919-1925 (2014).
Imamura S, Hagiwara D, Suzuki F, Kurano N, Harayama S. Genetic transformation of Pseudochoricystis ellipsoidea, an aliphatic hydrocarbon-producing green alga. J Gen Appl Microbiol. 58:1-10 (2012).
Ide T, Hoya M, Tanaka T, Harayama S. Enhanced production of astaxanthin in Paracoccus sp. strain N-81106 by using random mutagenesis and genetic engineering. Biochem Eng J 65:37-43 (2012).
Ito K, Wakai S, Tsurumaru H, Iino T, Mori K, Uchiyama T, Miki O, Harayama, S. Iron corrosion by methane-producing archaea (MPA) and sulfate-reducing bacteria (SRB) utilizing metallic iron as an electron donor. Corrosion Engineering 60: 336 (2011).
Alonso-Gutiérrez J, Teramoto M, Yamazoe A, Harayama S, Figueras A, Novoa B. Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain). J Appl Microbiol. 111:800-810 (2011).
Isobe K, Koba K, Ueda S, Senoo K, Harayama S, Suwa Y. A simple and rapid GC/MS method for the simultaneous determination of gaseous metabolites. J Microbiol Methods. 84:46-51 (2011).
Mori K, Harayama S.Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments. Int J Syst Evol Microbiol. 61:138-143 (2011).
Teramoto M, Ohuchi M, Hatmanti A, Darmayati Y, Widyastuti Y, Harayama S, Fukunaga Y. Oleibacter marinus gen. nov., sp. nov., a bacterium that degrades petroleum aliphatic hydrocarbons in a tropical marine environment. Int J Syst Evol Microbiol. 61:375-380 (2011).
Hamada M, Iino T, Iwami T, Harayama S, Tamura T, Suzuki K. Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov. J Gen Appl Microbiol. 56:427-436 (2010).
Kanaly RA, Harayama S. Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria. Microb Biotechnol. 3:136-164 (2010).
Teramoto M, Suzuki M, Hatmanti A, Harayama S. The potential of Cycloclasticus and Altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments. J Biosci Bioeng. 110:48-52 (2010).
Uchiyama T, Ito K, Mori K, Tsurumaru H, Harayama S. Iron-corroding methanogen isolated from a crude-oil storage tank. Appl Environ Microbiol. 76:1783-1788 (2010).
Iino T, Mori K, Uchino Y, Nakagawa T, Harayama S, Suzuki K. Ignavibacterium album gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of Ignavibacteria classis nov., for a novel lineage at the periphery of green sulfur bacteria. Int J Syst Evol Microbiol. 60:1376-1382 (2010).
Mori K, Tsurumaru H, Harayama S. Iron corrosion activity of anaerobic hydrogen-consuming microorganisms isolated from oil facilities. J Biosci Bioeng.110(4):426-30 (2010).
Kurahashi M, Fukunaga Y, Sakiyama Y, Harayama S, Yokota A. Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov. Int J Syst Evol Microbiol. 60:2314-2319 (2010).
Takeshi Terahara T, Yamada K, Kurata S, Yokomaku T, Tuneda S, Harayama S. Direct cloning and expression of putative esterase genes from environmental DNA. Enzyme and Microbial Technol 47:17-23 (2010).
Kanaly RA, Harayama S.
Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria.
Microbial Biotech., 2(3): 136-164 (2010).
Mori K, Tsurumaru H, Harayama S.
Iron corrosion activity of anaerobic hydrogen-consuming microorganisms isolated from oil facilities.
J Biosci Bioeng.,110(4), 426-430 (2010).
Teramoto M, Suzuki M, Hatmanti A,Harayama S.
The potential of Cycloclasticus and Altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments.
J Biosci Bioeng.,110(1): 48-52 (2010).
Teramoto M, Ohuchi M, Hatmanti A, Darmayati Y, Widyastuti Y, Harayama S, Fukunaga Y.
Oleibacter marinus gen. nov., sp. nov., a novel bacterium that degrades petroleum aliphatic hydrocarbons in the tropical marine environment.
Int J Syst Evol Microbiol.,61(2):375-80 (2010).
Mori K, Harayama S.
Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., novel mesophilic methanogens isolated from salty environments.
Int J Syst Evol Microbiol.,61(1):138-43 (2010).
Uchiyama T, Ito K, Mori K, Tsurumaru H, Harayama S.
Iron-corroding methanogen isolated from a crude-oil storage tank.
Appl Environ Microbiol.,76(6): 1783-1788 (2010).
Kurahashi M, Fukunaga Y, Sakiyama Y, Harayama S, Yokota A.
Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov.
Int J Syst Evol Microbiol.,60(10):2314-9 (2009).
Fukunaga Y, Kurahashi M, Sakiyama Y, Ohuchi M, Yokota A, Harayama S.
Phycisphaera mikurensis gen. nov., sp. nov., isolated from a marine alga, and proposal of Phycisphaeraceae fam. nov., Phycisphaerales ord. nov. and Phycisphaerae classis nov. in the phylum Planctomycetes.
J Gen Appl Microbiol.,55(4): 267-275 (2009).
Iino T, Mori K, Uchino Y, Nakagawa T, Harayama S, Suzuki K.
Ignavibacterium album gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of Ignavibacteria classis nov., for a novel lineage at the periphery of green sulfur bacteria.
Int J Syst Evol Microbiol.,60(6): 1376-1382 (2009).
Hamada M, Iino T, Tamura T, Iwami T, Harayama S, Suzuki K.
Serinibacter salmoneus gen. nov., sp. nov., an actinobacterium isolated from the intestinal tract of a fish, and emended descriptions of the families Beutenbergiaceae and Bogoriellaceae.
Int J Syst Evol Microbiol.,59(11): 2809-2814 (2009).
Khan ST, Harayama S, Tamura T, Ando K, Takagi M, Kazuo SY.
Paraoerskovia marina gen. nov., sp. nov., an actinobacterium isolated from marine sediment.
Int J Syst Evol Microbiol.,59(8): 2094-2098 (2009).
Teramoto M, Suzuki M, Okazaki F, Hatmanti A, Harayama S.
Oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment.
Microbiology.,155(10): 3362-3370 (2009).
Nakashima T, Anzai K, Kuwahara N, Komaki H, Miyadoh S, Harayama S, Tianero MD, Tanaka J, Kanamoto A, Ando K.
Physicochemical characters of a tyrosinase inhibitor produced by Streptomyces roseolilacinus NBRC 12815.
Biol Pharm Bull.,32(5): 832-836 (2009).
Kurahashi M, Fukunaga Y, Sakiyama Y, Harayama S, Yokota A.
Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.
Int J Syst Evol Microbiol., 59(4): 869-873 (2009).
Iino T, Suzuki K, Harayama S.
Lacticigenium naphtae gen. nov., sp. nov., a halotolerant and motile lactic acid bacterium isolated from crude oil.
Int J Syst Evol Microbiol., 59(4): 775-780 (2009).
Kurihara Y, Shirouzu , Tokumasu S, and Harayama S.
Hirsutella proturicola sp. nov. isolated from a proturan, Baculentulus densus (Protura, Hexapoda)
Mycoscience., Vol. 50(1): 56-62 (2009).
Fukunaga Y, Kurahashi M, Yanagi K, Yokota A, Harayama S.
Acanthopleuribacter pedis gen. nov., sp. nov., a marine bacterium isolated from a chiton, and description of Acanthopleuribacteraceae fam. nov., Acanthopleuribacterales ord. nov., Holophagaceae fam. nov., Holophagales ord. nov. and Holophagae classis nov. in the phylum 'Acidobacteria'.
Int J Syst Evol Microbiol., 58(11): 2597-2601 (2008).
Komaki H, Fudou R, Iizuka T, Nakajima D, Okazaki K, Shibata D, Ojika M, Harayama S.(2008)
PCR detection of type I polyketide synthase genes in myxobacteria.
Appl Environ Microbiol., 74(17): 5571-5574 (2008).
Iino T, Nakagawa T, Mori K, Harayama S, Suzuki K.
Calditerrivibrio nitroreducens gen. nov., sp. nov., a thermophilic, nitrate-reducing bacterium isolated from a terrestrial hot spring in Japan.
Int J Syst Evol Microbiol., 58(7): 1675-1679 (2008).
Khan ST, Nakagawa Y, Harayama S.
Fulvibacter tottoriensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment.
Int J Syst Evol Microbiol., 58(7): 1670-1674 (2008).
Anzai K, Ohno M, Nakashima T, Kuwahara N, Suzuki R, Tamura T, Komaki H, Miyadoh S, Harayama S, Ando K.
Taxonomic distribution of Streptomyces species capable of producing bioactive compounds among strains preserved at NITE/NBRC.
Appl Microbiol Biotechnol., 80(2): 287-295 (2008).
Koma D, Sawai T, Hara R, Harayama S, Kino K.
Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives.
Appl Microbiol Biotechnol., 79(5): 775-784 (2008).
Takarada H, Sekine M, Kosugi H, Matsuo Y, Fujisawa T, Omata S, Kishi E, Shimizu A, Tsukatani N, Tanikawa S, Fujita N, Harayama S.
Complete genome sequence of the soil actinomycete Kocuria rhizophila.
J Bacteriol., 190(12): 4139-4146 (2008).
Kurahashi M, Fukunaga Y, Harayama S, Yokota A.
Sneathiella glossodoripedis sp. nov., a marine alphaproteobacterium isolated from the nudibranch Glossodoris cincta, and proposal of Sneathiellales ord. nov. and Sneathiellaceae fam. nov.
Int J Syst Evol Microbiol., 58(3): 548-552 (2008).
Khan ST, Takaichi S, Harayama S.
Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay.
Int J Syst Evol Microbiol., 58(2): 383-386 (2008).
Yamada K, Terahara T, Kurata S, Yokomaku T, Tsuneda S, Harayama S.
Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids.
Environ Microbiol., 10(4): 978-987 (2008).
Kurihara Y, Machida R, Fukui M, Okuda T, and Harayama S.
Entomopathogenic fugi isolated from laboratory-reared Baculentulus densus (Acerentomidae, Protura).
Edaphologia80: 25-28 (2007).
Urai M, Yoshizaki H, Anzai H, Ogihara J, Iwabuchi N, Harayama S, Sunairi M, and Nakajima M.
Structural analysis of an acidic, fatty acid ester-bonded extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.
Carbohydr. Res., 342(7): 933-942 (2007).
Urai M, Yoshizaki H, Anzai H, Ogihara J, Iwabuchi N, Harayama S, Sunairi M, Nakajima M.
Structural analysis of mucoidan, an acidic extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.
Carbohydr. Res., 342(7): 927-932 (2007).
Khan ST, Nakagawa Y, and Harayama S.
Sediminibacter furfurosus gen. nov., sp. nov. and Gilvibacter sediminis gen. nov., sp. nov., novel members of the family Flavobacteriaceae.
Int. J. Syst. Evol. Microbiol., 57(2): 265-269 (2007).
Khan ST, Nakagawa Y, and Harayama S.
Galbibacter mesophilus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae.
Int. J. Syst. Evol. Microbiol., 57(5): 969-973 (2007).
Khan ST, Harayama S.
Paraferrimonas sedimenticola gen. nov., sp. nov., a marine bacterium of the family Ferrimonadaceae.
Int. J. Syst. Evol. Microbiol., 57(7): 1493-1498 (2007).
Ueda JY, Hashimoto J, Nagai A, Nakashima T, Komaki H, Anzai K, Harayama S, Doi T, Takahashi T, Nagasawa K, Natsume T, Takagi M, and Shin-ya K.
New aureothin derivative, alloaureothin, from Streptomyces sp. MM23.
J. Antibiot., (Tokyo). 60(5): 321-324(2007).
Yamamura H, and Harayama S.
Method for selective isolation of Mycobacteria using olive oil emulsified with SDS.
Biosci. Biotechnol. Biochem., 71(6): 1553-1556 (2007).
Yamamura H, Tamura T, Sakiyama Y, and Harayama S.
Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan.
Int. J. Syst. Evol. Microbiol., 57(7): 1599-1602 (2007).
Khan ST, Nakagawa Y, and Harayama S.
Sediminitomix flava gen. nov., sp. nov., of the phylum Bacteroidetes, isolated from marine sediment.
Int. J. Syst. Evol. Microbiol., 57(8): 1689-1693 (2007).
Iino T, Mori K, Tanaka K, Suzuki K, and Harayama S.
Oscillibacter valericigenes gen. nov., sp. nov., a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam.
Int. J. Syst. Evol. Microbiol., 57(8): 1840-1845 (2007).
Sawai T, Koma D, Hara R, Kino K, and Harayama S.
A high-throughput and generic assay method for the determination of substrate specificities of thermophilic alpha-aminotransferases.
J . Microbiol. Methods., 71(1): 32-38 (2007).
Ueda JY, Togashi T, Matukura S, Nagai A, Nakashima T, Komaki H, Anzai K, Harayama S, Doi T, Takahashi T, Natsume T, Kisu Y, Goshima N, Nomura N, Takagi M, and Shin-Ya K.
A novel nuclear export inhibitor JBIR-02, a new piericidin discovered from Streptomyces sp. ML55.
J. Antibiot., (Tokyo) 60(7): 459-462 (2007).
Khan ST, Fukunaga Y, Nakagawa Y, and Harayama S.
Emended descriptions of the genus Lewinella and of Lewinella cohaerens, Lewinella nigricans and Lewinella persica, and description of Lewinella lutea sp. nov. and Lewinella marina sp. nov.
Int. J. Syst. Evol. Microbiol., 57(12): 2946-2951 (2007).
Balk SH, Yoshioka H, Yukawa H, and Harayama S.
Synthesis of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) with thermostabilized low-specific L-threonine aldolase from Streptomyces coelicolor A3(2).
J. Microbiol. Biotechnol., 17(5): 721-727 (2007).
Khan ST, Nakagawa Y, and Harayama S.
Sediminibacter furfurosus gen. nov., sp. nov. and Gilvibacter sediminis gen. nov., sp. nov., novel members of the family Flavobacteriaceae.
Int. J Syst. Evol. Microbiol., 57(2): 265-269 (2007).
Harayama S, and Kasai H.
Bacterial phylogeny reconstruction from molecular sequences, In: Molecular Identification, Systematics, and Population Structure of Prokaryotes
Stackebrandt, E., ed. Birkhause.: 105-140 (2006)
Peng X, Taki H, Komukai S, Sekine M, Kanoh K, Kasai H, Choi SK, Omata S, Tanikawa S, Harayama S, and Misawa N.
Characterization of four Rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols.
Appl. Microbiol. Biotechnol., 71(6): 824-832 (2006).
Sekine M, Tanikawa S, Omata S, Saito M, Fujisawa T, Tsukatani N, Tajima T, Sekigawa T, Kosugi H, Matsuo Y, Nishiko R, Imamura K, Ito M, Narita H, Tago S, Fujita N, and Harayama S.
Sequence analysis of three plasmids harboured in Rhodococcus erythropolis strain PR4.
Environ. Microbiol., 8(2): 334-346 (2006).
Fukunaga Y, Kurahashi M, Tanaka K, Yanagi K, Yokota A, and Harayama S.
Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts).
Int. J. Syst. Evol. Microbiol., 56(2): 343-347 (2006).
Khan ST, Nakagawa Y, and Harayama S.
Krokinobacter gen. nov., with three novel species, in the family Flavobacteriaceae.
Int. J. Syst. Evol. Microbiol., 56(2): 323-328 (2006).
Urai M, Anzai H, Ogihara J, Iwabuchi N, Harayama S, Sunairi M, and Nakajima M.
Structural analysis of an extracellular polysaccharide produced by Rhodococcus rhodochrous strain S-2.
Carbohydr. Res., 341(6): 766-775 (2006).
Khan ST, Nakagawa Y, and Harayama S.
Sediminicola luteus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae.
Int. J. Syst. Evol. Microbiol., 56(4): 841-845 (2006).
Khan ST, Nakagawa Y, and Harayama S.
Sandarakinotalea sediminis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae.
Int. J. Syst. Evol. Microbiol., 56(5): 959-963 (2006).
Nakagawa T, Iino T, Suzuki K-I, and Harayama S.
Ferrimonas futtsuensis sp. nov. and Ferrimonas kyonanensis sp. nov., selenate-reducing bacteria belonging to the Gammaproteobacteria isolated from Tokyo Bay.
Int. J Syst. Evol. Microbiol., 56(11): 2639-2645 (2006).
Koma D, Sawai T, Harayama S, and Kino K.
Overexpression of the genes from thermophiles in Escherichia coli by high-temperature cultivation.
Appl. Microbiol. Biotechnol., 73(1): 172-180 (2006).
Takaichi S, Maoka T, Akimoto N, Khan ST, and Harayama S.
Major carotenoid isolated from Paracoccus schoinia NBRC 100637T is adonixanthin diglucoside.
J. Nat. Prod., 69(12): 1823-1825 (2006).
Komaki H, and Harayama S.
Sequence diversity of type-II polyketide synthase genes in Streptomyces.
Actinomycetologica., 20: 42-48 (2006).
バイオレメディエーション
バイオレメディエーションとは、生物の力を利用して環境汚染物質を除去する技術のことを言います。当研究室では、現地スタッフを雇用し、実際にインドネシアのジャカルタ湾に位置するパリ島の礁湖で、原油が漂着した砂浜海岸に対するバイオレメディエーションの効果を定量的に把握するための大規模な実証試験を行いました。
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